Import Question JSON

Current Question (ID: 16234)

Question:

The DNA fragments separated on an agarose gel can be visualised after staining with

Options:

1. bromophenol blue
2. acetocarmine
3. aniline blue
4. ethidium bromide

Solution:

The DNA fragments separated on an agarose gel can be visualised after staining with ethidium bromide. It is intercalating agent and a fluorescent agent. The stained DNA fragments are seen bright orange coloured band under UV-light. Thinking process Intercalation is the insertion of molecules between the planar bases of DNA. This process is used as a method for analysing DNA. Intercalation occurs, when ligands of an appropriate size and chemical nature fit themselves in between base pairs of DNA. These ligands are mostly polycyclic, aromatic and planar and therefore often make good nucleic acid stains. Intensively studied DNA intercalator include ethidium bromide, proflavine, etc.

Import JSON File

Upload a JSON file containing LaTeX/MathJax formatted question, options, and solution.

Expected JSON Format:

{
  "question": "The mass of carbon present in 0.5 mole of $\\mathrm{K}_4[\\mathrm{Fe(CN)}_6]$ is:",
  "options": [
    {
      "id": 1,
      "text": "1.8 g"
    },
    {
      "id": 2,
      "text": "18 g"
    },
    {
      "id": 3,
      "text": "3.6 g"
    },
    {
      "id": 4,
      "text": "36 g"
    }
  ],
  "solution": "\\begin{align}\n&\\text{Hint: Mole concept}\\\\\n&1 \\text{ mole of } \\mathrm{K}_4[\\mathrm{Fe(CN)}_6] = 6 \\text{ moles of carbon atom}\\\\\n&0.5 \\text{ mole of } \\mathrm{K}_4[\\mathrm{Fe(CN)}_6] = 6 \\times 0.5 \\text{ mol} = 3 \\text{ mol}\\\\\n&1 \\text{ mol of carbon} = 12 \\text{ g}\\\\\n&3 \\text{ mol carbon} = 12 \\times 3 = 36 \\text{ g}\\\\\n&\\text{Hence, 36 g mass of carbon present in 0.5 mole of } \\mathrm{K}_4[\\mathrm{Fe(CN)}_6].\n\\end{align}",
  "correct_answer": 4
}