Import Question JSON

Current Question (ID: 16255)

Question:

Gel electrophoresis is used for

Options:

1. cutting of DNA into fragments
2. separation of DNA fragments according to their size
3. construction of recombinant DNA by joining with cloning vectors
4. isolation of DNA molecule

Solution:

When genomic DNA extracted from any tissue of a plant or animal species is digested with a restriction enzyme, it is cleaved into segments. The segments of different sizes can be separated through gel electrophoresis. Gel electrophoresis involves the movement of fragments from a well created on one edge of the gel. Endonucleases are enzymes that produce internal cuts called cleavage in DNA molecules. A class of endonucleases cleaves DNA only within or near those sites, which have specific base sequences, such endonucleases are known as restriction endonucleases. The selected vector is cut open with a restriction enzyme that has a unique target site located in the sequence where the DNA insert should be integrated to facilitate the selection of recombinant DNA. PAGE 198, XII NCERT

Import JSON File

Upload a JSON file containing LaTeX/MathJax formatted question, options, and solution.

Expected JSON Format:

{
  "question": "The mass of carbon present in 0.5 mole of $\\mathrm{K}_4[\\mathrm{Fe(CN)}_6]$ is:",
  "options": [
    {
      "id": 1,
      "text": "1.8 g"
    },
    {
      "id": 2,
      "text": "18 g"
    },
    {
      "id": 3,
      "text": "3.6 g"
    },
    {
      "id": 4,
      "text": "36 g"
    }
  ],
  "solution": "\\begin{align}\n&\\text{Hint: Mole concept}\\\\\n&1 \\text{ mole of } \\mathrm{K}_4[\\mathrm{Fe(CN)}_6] = 6 \\text{ moles of carbon atom}\\\\\n&0.5 \\text{ mole of } \\mathrm{K}_4[\\mathrm{Fe(CN)}_6] = 6 \\times 0.5 \\text{ mol} = 3 \\text{ mol}\\\\\n&1 \\text{ mol of carbon} = 12 \\text{ g}\\\\\n&3 \\text{ mol carbon} = 12 \\times 3 = 36 \\text{ g}\\\\\n&\\text{Hence, 36 g mass of carbon present in 0.5 mole of } \\mathrm{K}_4[\\mathrm{Fe(CN)}_6].\n\\end{align}",
  "correct_answer": 4
}